Enhanced bacterial expression of several mammalian cytochrome P450s by codon optimization and chaperone coexpression
نویسندگان
چکیده
منابع مشابه
Cytochrome P450s and chemoprevention.
The cytochrome P450 mono-oxygenase system represents a major defence against chemical challenge from the environment, constituting part of an adaptive response mounted by an organism following exposure to harmful agents. Cytochrome P450s are also able to catalyse the activation of compounds to toxic products, and participate in a variety of essential 'housekeeping' functions, such as biosynthes...
متن کاملThe Cytochrome P450s
* Author for correspondence: Fatma M.A. El-garj, School of Distance Education, Universiti Sains Malaysia, 11800 Penang, Malaysia. Email [email protected]. Abstract. Cytochrome P450 monooxygenases are versatile biocatalysts that incorporate oxygen into an enormous range of molecules. The cytochrome P450 proteins are involved in the catalysis of different reactions and these properties have bee...
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a:4:{s:10:"Background";s:897:"Human luteinizing hormone (hLH) belongs to glycoprotein hormones which is composed of two non-covalently linked subunit, α and β. The α-subunit is similar in all glycoprotein hormones, whereas the β-subunit is conferring the hormonal specificity. This hormone has important roles in the growth and maturity of sexual organs and secondary sexual characteristics and st...
متن کاملA world of cytochrome P450s.
The world we live in is a biosphere influenced by all organisms who inhabit it. It is also an ecology of genes, with some having rather startling effects. The premise put forth in this issue is cytochrome P450 is a significant player in the world around us. Life and the Earth itself would be visibly different and diminished without cytochrome P450s. The contributions to this issue range from ev...
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Activin A is a member of the transforming growth factor β super family. Because of its extensive clinical usages, its recombinant production is beneficial. In this study, activin A was expressed in E. coli using the pET 21a expression vector. The optimization of the activin A production in E. coli was done by using the response surface methodology (RSM). At this stage, the effect of IPTG and la...
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ژورنال
عنوان ژورنال: Biotechnology Letters
سال: 2009
ISSN: 0141-5492,1573-6776
DOI: 10.1007/s10529-009-0059-5